Poster Authors and Abstracts – CSU Annual Biotechnology Symposium. CSU Biotechnology Symposium. Posters with Author Listings and Abstracts. Use “Find” function or Ctrl F to search. Poster #: 1 Campus: CSU Northridge. Poster Category: Agriculture/Biofuels/Environment. Keywords: Arabidopsis thaliana, Genetic engineering, Weather pollution. Project Title: Overexpression of an aminocyclopropane- 1- carboxylic acid synthase gene in Arabidopsis thaliana and its effect on removing environmental pollutants. Author List: Ramadoss, Niveditha; Graduate, Biology, California State University, Northridge, Presenting Author. Gupta, Dinesh; Washington University, St. Louis. Guenther, Alex; University of California, Irvine. Basu, Chhandak; Biology, California State University, Northridge. Abstract: Rise in air pollution has led to increasing cancer, several respiratory and cardiac illnesses in humans. World Health Organization estimates that one in eight person die from air pollution. The main objective of our study is to analyze the feasibility of genetically engineered plants to remove polluting volatile compounds from air. To achieve this goal we genetically transformed Arabidopsis thaliana with an ACS (Aminocyclopropane- 1- carboxylic acid synthase) gene. Using quantitative real- time PCR (q. PCR), we previously confirmed that the ACS gene was upregulated in poplar (Populus trichocarpa) plants when exposed to environmental pollutants including ozone and methyl vinyl ketone. Therefore, we hypothesize that ACS gene might play an important role in poplar plants in mitigating stresses resulting from pollutant exposure. The homolog of poplar ACC synthase gene was PCR amplified from Arabidopsis thaliana and cloned into p. BINmgfp. 5- er vector, featuring a constitutive cauliflower mosaic virus promoter removing the native jellyfish green fluorescent (GFP) gene. The recombinant plasmid containing the ACS gene was electroporated into the GV1. Agrobacterium tumefaciens. Latest edition of CJS Professional online in full. Noteworthy this month: How do you connect with CJS? We want to know the best way to share our information with you. Arabidopsis plants were genetically engineered with ACS gene by dipping the T0 (transgenic zero or wild type) flowering plants into GV1. Agrobacterium containing the recombinant binary vector. A Google ingyenes szolgáltatása azonnal lefordítja a szavakat, kifejezéseket és weboldalakat a magyar és 100 további nyelv kombinációjában. 2017 CSU Biotechnology Symposium Posters with Author Listings and Abstracts. Use “Find” function or Ctrl F to search. Poster #: 1 Campus: CSU Northridge. Type or paste a DOI name into the text box. Your browser will take you to a Web page (URL) associated with that DOI name. Send questions or comments to doi. The transformed plants were selected through kanamycin resistance and grown till T3 (third transgenic) generation. The ACS gene expression of these plants will be confirmed through q. PCR. The pollutant absorbing capacity will be tested by placing the transformed T3 plants in an environmental gas chamber where they will be exposed to various levels of pollutants. The ability of the transformed plants to absorb pollutants will be evaluated by performing gas chromatography mass spectrometry analyses to measure pollutants in the inlet and outlet airflow in order to quantify the pollutant deposition rate. Our use of a model plant Arabidopsis is . Our future goal will be to transform tree species and evaluate their potentials to remove pollutants. These studies will open doors for use of transgenic plants to clean up environments. Poster #: 2 Campus: Sonoma State University. Poster Category: Agriculture/Biofuels/Environment. Keywords: Biosensors, Wireless Biosensor Network , Soil. Project Title: A Comparative Study of RF Wave Attenuation in Soil and Sand. Author List: Palmerin, Abraham; Undergraduate, Engineering Science , Sonoma State University, Presenting Author. Farahmand, Farid; Engineering Science, Sonoma State University. Fong, Erin; Lawrence Berkeley National Laboratory, Engineering Division. Abstract: Soil is the most spatially complex stratum of a terrestrial ecosystem. Therefore, soil monitoring and understanding changes in soil ecosystem are considered to be important aspects of any environmental monitoring. The recent emergence of Wireless Biosensor Networks (WBNs) promises addressing many ecological questions, particularly related to soil, though providing uninterrupted real- time data from various biosensors using RF signals. In this work we focused on understanding the impact of soil composition (e. RF waves travel underground. This study is crucial in order to define the appropriate RF band, power level, and RF antenna size for extended underground monitoring. Our research focused on 5. GHz ISM bands. The first part of our experiment was conducted in a 1. We measured the RF signal path loss through the sand for the above frequencies at different horizontal distances between the transmitter and receiver. We also measured RF signal strength as we changed the vertical distances between the two antennas. In the second part of our experiment, we used regular gardening soil mostly composed of organic matter, perlite and vermiculite. Vermiculite keeps the soil hydrated and is part of the clay minerals group. We made similar measurements. In both cases, we maintained the temperature at 2. Celsius. We normalized the obtained path loss measurements as a function of distance for all frequencies to remove any offset difference due to factors such as antennas. Our results indicate that at 0. GHz the received signal power level decays with distance by about 1. B/cm when traveling in soil. We also found that at higher frequencies, the RF signal experiences 4- 6 d. B more attenuation in soil than air. The exact value depends on the frequency and antenna polarization type. On the other hand, the path loss in sand is about 0. B more than air. It is therefore, preferred to use lower band frequencies for underground biosensors. However, the trade off will be antenna size and its design complexity. Our results conclude that it is critical to carefully manage signal power and understand antenna design when wireless biosensors are placed in an area with high quantity of clay content. This study was carried out in collaboration with Lawrence Berkeley National Laboratory, Division of Earth Sciences. Poster #: 3 Campus: CSU Long Beach. Poster Category: Agriculture/Biofuels/Environment. Keywords: Arabidopsis, Senescence, Epigenetics. Project Title: A TIR- NBS- LRR Class Disease Resistance Protein Mays be a Positive Regulator of Developmental Senescence in Arabidopsis thaliana. Author List: Mendoza, Victor ; Undergraduate, Chemistry & Biochemistry, California State University, Long Beach, Presenting Author. Brusslan, Judy; Biological Sciences, California State University, Long Beach. Abstract: Leaf senescence is the final stage in leaf development in which the leaf reallocates its nutrients to growing parts of the plant. This study aims to determine if the At. TIR- NBS- LRR class disease resistance protein (DRP), is involved in leaf senescence in Arabidopsis thaliana. This gene is up- regulated in leaf senescence and the increase in expression occurs in parallel to increases in the H3. K4me. 3 mark. Two distinct transfer- DNA (T- DNA) insertion lines that disrupt At. DNA sequencing. Loss of gene expression was confirmed by amplification of c. DNA using insertion- specific primers. Chlorophyll and total protein levels were used as physical markers for age. Differences between wild type and mutant leaves were quantified and compared using two- tailed student’s t- test. The transcript levels of robust senescence- up regulated genes (WRKY7. NIT2), were also compared. Our results revealed a significant increase of protein levels in the k. A trend of increased chlorophyll levels in k. Quantitative real- time PCR data revealed no trends of increased senescence associated gene transcript levels. We infer that the TIR- NBS- LRR, from the TNL- H subfamily, K4- DRP, may be involved in the protein degradation signaling cascade in senescence. Further work includes generating triple mutants of the TNL- H class genes that are up- regulated during senescence and marked by H3. K4me. 3. A strong delayed- senescence phenotype in triple TNL- H mutants would suggest that the TNL- H subfamily of genes may be positive regulators of senescence. Poster #: 4 Campus: CSU Fresno. Poster Category: Agriculture/Biofuels/Environment. Keywords: fleabane, herbicide resistance, greenhouse common garden. Project Title: Fitness cost of glyphosate resistance in hairy fleabane (Erigeron bonariensis) under drought conditions. Author List: Pathak, Ankit; Graduate, Biology, California State University, Fresno, Presenting Author. Waselkov, Katherine; Biology, California State University, Fresno. Abstract: Many agriculturally invasive, weedy plant species have evolved resistance various chemical herbicides, in large part because of continuous application of these chemicals to agricultural environments. Glyphosate is a powerful, low- toxicity herbicide that has been extensively applied to crops in the San Joaquin Valley of California since the 1. In 2. 00. 7, a glyphosate- resistant population of hairy fleabane (Erigeron bonariensis) was discovered on a California roadside, and since then, glyphosate resistance in fleabane has become widespread in the San Joaquin Valley. During the same time period (2. California agriculture. Since this weed species competes with young fruit trees and vines for resources, and as water resources become more limited due to climatic changes, our lab has begun to study the interaction between glyphosate resistance (GR) and drought stress in hairy fleabane. Our hypothesis was that glyphosate resistance has no fitness cost under drought- stressed conditions. Twenty plants each from a well- characterized GR fleabane population and a glyphosate- sensitive (GS) population were grown in a greenhouse environment: half of the plants of each biotype were exposed to drought stress conditions (4. We measured different fitness components, including height and leaf number over time, days to bolting and flowering, above- ground and below- ground biomass, and seed production. Treatment had a stronger effect than biotype on all measurements that showed significant differences between groups, including days to flowering (8.
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